BioXplorer 400P High-Pressure Benchtop, Parallel 4 Bioreactor Platform

The BioXplorer 400P is an automated parallel pressure bioreactor system designed by H.E.L, for the discovery and optimization of barophilic strains and generally fermentation requiring a high gas transfer rate. Built for applications ranging from syngas fermentation to cell-free enzymatically catalyzed processes, the BioXplorer 400P uses pressure and precision gas control to accelerate fermentation that depend on the availability of dissolved gases. The customizable configuration combined with versatile software allows the BioXplorer 400P to fulfill your precise needs, giving you the data you need to scale up faster a fermentation culture model.

Key Highlights:

Pressurized Bioprocessing

The BioXplorer 400P supports up to four parallel fermentation at up to 10 bar pressure — a capability that is unique among benchtop fermentation platforms available. Pressurized systems increase the availability of dissolved gases and accelerate gas-dependent fermentation, making the BioXplorer 400P an ideal tool for process optimization and its impact on scale-up. Engineered with a focus on safety and ease of use, the system features 2 gas spargers per reactor to minimize risks associated with potentially hazardous gas mixtures. Adding pressure to improve a fermentation model can deliver higher yields without compromising functionality and configurability.

Configurable System

The BioXplorer 400P uses a modular design and can be supplied with a wide range of sensors and probes, gas and liquid addition, enabling it to be tailored to your fermentation requirements. This maximizes functionality and ease of use while minimizing bench space requirements and cost.

Flexibility to Optimize Processes

The choice of sensors and probes to monitor and control a wide range of physicochemical parameters provides in-depth knowledge. Using H.E.L's WinISO for tight control and responsiveness, full automatization of the process is achievable. The BioXplorer 400P can operate 4 fermenters in parallel or independently, reducing the optimization time of a fermentation to develop a culture model

Specifications

• Number of independent parallel fermenters: 4
• Bioreactor material: 316L S/S headplate and fermenter vessel 
• Total volume: 500 ml
• Working volume: 120–400 ml
• Pressure rating: Up to 10 bar
• Sterilization: Autoclavable 
• Temperature range: 0–135°C
• Temperature resolution: 0.1°C
• Agitation: Bottom magnetically driven or top agitation (magnetically coupled)
• Stirrer speed:
  • Bottom drive: 250–1500 rpm
  • Top agitation: 20–2000 rpm
• Liquid addition per fermenter: 2 and up to 2 additional feeding loops
• Gas additions: 2 sets of 4 mass flow controllers allowing independent control for each fermenter as standard; option for 2 additional sets
• Standard options for MFC calibration: Air, oxygen, nitrogen, hydrogen, carbon monoxide, carbon dioxide, or methane
• Gas spargers: 2
• Exhaust gas: Peltier cooled condenser
• pH control: 1-way control (2-way control optional)
• pH range: 0–14
• Polarographic DO sensing: Optional
• DO control: Gas/liquid flow rate, cascade mode
• BioVIS – cell density trend monitoring without sampling: Optional
• Foam/level probe: Optional
• Control PC and software: Windows® PC with WinISO control software preinstalled

WinISO Control Software

• Fully independent control of each fermenter
• Advanced control strategies for agitation and liquid and gas additions based on feedback from sensors, time-based profiles, or event detection
• Real-time display and logging of all reaction parameters
• Easy export of data in a range of text formats, including .csv

*The upper temperature limit for data acquisition from standard DO probes is 40°C. Contact your local API representative to discuss alternative DO probes for use at elevated temperatures. The lower temperature limit requires the integrated circulator option (chiller)