BioXplorer 100 Benchtop Parallel 8 Bioreactor Platform

The BioXplorer 100 is a multi-bioreactor system designed by H.E.L for the optimization of aerobic fermentations. With a working volume as low as 50 ml, the BioXplorer 100 allows for low-working volume processes. The design of the bioreactor's lid enables customizable configurations, choosing from a wide range of sensors, probes and feed options. Controlled by WinISO, the BioXplorer 100 is designed to be a fully automated system.

Key Highlights:

Customizable Configurations

The BioXplorer 100 features 8 configurable bioreactors with working volumes ranging from 50 to 150 ml, controlled independently or in parallel with a versatile range of probes and sensor configuration. Its modular design allows precise additions of liquids and gases via up to four sets of eight peristaltic pumps and up to two sets of mass flow controllers.

Optimized Solutions for a Range of Applications

The BioXplorer 100 is optimized for aerobic fermentation and optimal growth, providing more in-depth process insights and comprehensive data. Eight parallel fermenters enable simultaneous screening and high throughput of multiple processes. Paired with WinISO, real-time data acquisition allows live monitoring and control of a fermentation batch through feedback loops.

Flexibility and Control

Equipped with a highly customizable range of sensors, the BioXplorer 100 enables high-throughput data acquisition in real-time. Controlled by H.E.L's WinISO, the system allows strict control of bioprocess parameters, with automatization based on inline measurements enabling dynamic response to changes in the fermenter through feedback loops.

Compact Parallel Bioreactors

Space and cost-efficient, the BioXplorer 100 supports up to eight parallel fermentation cultures within a small overall footprint. Its low working volume minimizes reagent costs and fermentation times, while its flexible sensor configuration enables real-time data collection — an intelligent solution to overcome microbial bioprocessing challenges for screening, cloning or even a design of experiments for media optimization. 

Specifications

• • Number of independent parallel bioreactors: 8
  • Bioreactor material: Borosilicated glass, 316L S/S headplate
  • Min. and Max. culture volume: 50–150 ml
  • Sterilization: Autoclavable 
  • Temperature range: 0–130°C
  • Temperature resolution: 0.1°C
  • Agitation: Magnetic drive
  • Agitation range: 250–1500 rpm
  • Liquid additions per fermenter: 2 and up to 2 additional feeding loops
  • Gas additions: 1 set of 8 mass flow controllers allowing independent control for each fermenter; and 1 additional gas circuitry (optional)
  • Standard options for MFC calibration: Air; oxygen as an option
  • Gas spargers: 1
  • Exhaust gas: Peltier cooled condenser 
  • pH control: 1-way control (2-way control optional)
  • pH range: 0–14
  • Polarographic DO sensing: Standard
  • DO control: Gas/liquid flow rate, cascade mode 
  • BioVIS – cell density trend monitoring without sampling: Optional
  • Foam/level probe: Optional
  • Control PC and software: Windows® PC with WinISO control software preinstalled

WinISO Control Software

• Fully independent control of each fermenter
• Advanced control strategies for agitation control and liquid and gas additions based on feedback from sensors, time-based profiles, or event detection
• Real-time display and logging of all reaction parameters
• Easy export of data in a range of text formats, including .csv

*The upper temperature limit for data acquisition from standard DO probes is 40°C. Contact your local API representative to discuss alternative DO probes for use at elevated temperatures. The lower temperature limit requires the integrated circulator option (chiller).