BioXplorer 5000 Lab-Scale Bioreactor Platform
The BioXplorer 5000 and 5000P are fermentation units designed by H.E.L, ideally suited to drive scale-up efficiency. These systems are highly automated and highly customizable. The BioXplorer 5000P is the ideal choice for pressure applications up to 10 bar.
Key Features:
The BioXplorer 5000 and 5000P are suitable for a wide range of applications including syngas fermentation and cell-free biocatalysis, with:
- Automated inventory monitoring
- BioVIS optical probe to monitor total cell density trends
- Choice of vessel sizes and types, including elevated pressure designs (up to 10 bar)
- Fully automated control
Software
The H.E.L control software runs the BioXplorer 5000 system, enabling complex, fully automated experiments to run unattended. This enables:
- Full fermentation control with unlimited step recipe planning; plans can be changed online at any time without process interruption
- Individual fermenter temperature and agitation control
- User-defined data-logging with customizable tabular and graphical displays
- Export data functions and integration with in-house electronic lab notebooks and record-keeping systems
- DoE and QbD package integration
The software controls a wide range of system features, including:
- Temperature
- Gas feeds, flow, and mix
- pH
- Dissolved oxygen
- Redox
- Agitation rate
- Torque (for viscosity indication)
- Pressure
- Liquid feed rates
- Antifoam and level detection
Options
Gas Feeds
- Choice of simple or more sophisticated gas flow control regimes
- Fermenter can be configured with single or multiple mass flow controllers
- Microbial fermentation with automatic oxygen enrichment
- dCO₂ can be used for pH control
- Ratios of several gases can be mixed and regulated with online feedback from off-gas analytics
Liquid Feeds
- Choice of liquid feeds for standard and pressure applications
- High pressure metering pumps enable exceptional pH and dosing control as well as a wide operating range
pH, Dissolved Oxygen, and Redox
- A wide range of probes are available to cater for different fermentation types, vessel sizes, and applications
- All sensor and probe signals are integrated into the WinISO software to allow automated monitoring and feedback loops
Off-Gas Analysis
- Tandem off-gas analysis units monitor and control a culture process continuously
- Enables calculation of OUR, CER and RQ carbon dioxide production rate (CPR), and respiration quotient (RQ)
Cell Density and Biomass Monitoring
- BioVIS enables online monitoring of cell growth rate and biomass growth, reducing requirements for online sampling
- Capacitance viable cell density probes can also be integrated for VCD monitoring and control