BioXplorer 400XL Expanded Benchtop, Parallel 8 Bioreactor Platform

The BioXplorer 400XL is a multi-bioreactor system designed by H.E.L, featuring eight 500 ml fermenters for  fast optimization of aerobic fermentation culture models. The parallel capabilities of the BioXplorer 400XL, combined with the customizable configuration of the fermenter headplate, enables real-time measurement of selected parameters. Combined with WinISO, the BioXplorer 400XL is able to respond to rapid changes during the fermentation, allowing for complete automatization of the culture model.

Key Highlights:

Enhanced Bioreactor System

The BioXplorer 400XL features eight fermenters, each optimized for aerobic fermentation with working volumes of 120–400 ml. Equipped with a wide variety of probes and sensors, and offering multiple gas and liquid feed configurations, the system's ability to precisely control and monitor up to eight cultures in parallel greatly accelerates screening, characterization and process optimization leading to better understanding future scale-up challenges. 

Flexibility and Control

The BioXplorer 400XL combines larger working volumes with the replication and parallelization capabilities of eight independent fermenters controlled by WinISO. The software monitors analytical parameters of interest while maintaining stringent control to preserve ideal conditions within each fermenter. The flexibility of customizable liquid and gas pumps and agitation options makes it a powerful tool for the characterization and optimization of various fermentation models.

Specifications

• Number of independent parallel fermenters: 8
• Bioreactor material: Borosilicated glass, 316L S/S headplate
• Total volume: 500 ml
• Working volume: 120–400 ml
• Sterilization: Autoclavable
• Temperature range: 0–130°C
• Temperature resolution: 0.1°C
• Agitation:
  • Magnetically driven: 250–1500 rpm
  • Overhead agitation: 20–2000 rpm
• Liquid additions per fermenter: 2 and up to 2 additional feeding loops
• Gas additions: 1 set of 8 mass flow controllers allowing independent control for each fermenter; and 1 additional gas circuitry (optional)
• Standard options for MFC calibration: Air; oxygen as an option
• Gas spargers: 1
• Exhaust gas: Peltier cooled condenser
• pH control: 1-way control (2-way control optional)
• pH range:  0–14
• Polarographic DO sensing: Standard
• DO control: Gas/liquid flow rate, stirrer speed (optional)
• BioVIS – cell density trend monitoring without sampling: Optional
• Foam/level probe: Optional
• Control PC and software: Windows® PC with WinISO control software preinstalled

WinISO Control Software

• • Fully independent control of any fermenter
  • Advanced control strategies for agitation and liquid and gas additions based on feedback from sensors, time-based profiles, or event detection
  • Real-time display and logging of all reaction parameters
  • Easy export of data in a range of text formats, including .csv

• *The upper temperature limit for data acquisition from standard DO probes is 40°C. Contact your local API representative to discuss alternative DO probes for use at elevated temperatures. The lower temperature limit requires the integrated circulator option (chiller)